Biological Experiment Report "Observing Mitosis of Plant Cells"

First, the purpose of the experiment
1. Observe the process of mitosis in plant cells and identify different periods of mitosis.
2. Initially master the skills of making onion apical mitotic loading.
3. Initially master the method of drawing biographs.
Second, the experimental principle In the plant, mitosis is common in the apical, stem tip and other meristematic cells, the process of mitosis of higher plant cells, divided into the interphase, metaphase and early, middle, late, and late stages. The process of mitosis of plant cells can be observed with a high power microscope, and the period of mitosis of the cells is recognized according to the change of chromosomes in the cells at various times, and the chromosomes in the nucleus are easily colored by the basic dye.
Third, materials, onion tips, microscopes, slides, coverslips, droppers, tweezers, petri dishes, pencils, 15% by mass of hydrochloric acid,
95% alcohol, gentian violet with a mass fraction of 0.01g/ml, experimental procedure
1. Onion root tip culture
2. Dissociation: 5min
3. Rinse: 10min
4. Dyeing: 5min
5. Production
6. Microscopic examination

Five, pay attention to
1. Detachment is a necessary condition for the success of the experiment. The dissociation is sufficient, the organization can be dispersed, and the cells do not overlap.
2 . The rinsing time must be sufficient, otherwise the cells will not be dyed.
3 . When dyeing, the concentration of the dye solution and the dyeing time must be well mastered. In particular, the staining should not be too deep, otherwise the mirror will be purple and cannot be observed.

Six, discussion
1. What is the key to making onion apical mitotic sheets? Talk about your own experience.

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2. After observing the cells of each stage of mitosis, a schematic diagram of mitosis of the root tip cells was drawn and the period was indicated.