First biological experiment report
Experiment Exploring the hydrolysis of starch and sucrose by amylase I. Experimental purposes
1. Initially learn how to catalyze specific chemical reactions by enzymes.
2. Explore whether amylase can only catalyze specific chemical reactions.
Second, the experimental principle Starch and sucrose are non-reducing sugars, they can be hydrolyzed into reducing sugars under the catalytic action of enzymes, reducing sugars can be redox reaction with Feilin reagent to form brick red cuprous oxide precipitate.
The starch solution and the sucrose solution were respectively catalyzed by amylase, and the presence or absence of reducing sugar in the solution was determined by using the Ferien reagent, and it can be seen whether the amylase can catalyze the two chemical reactions.
3. Material utensils, dropper, test tube, match, test tube rack, thermometer, tripod, asbestos net, alcohol lamp, beaker, fresh amylase solution with mass fraction of 2%, soluble starch solution with mass fraction of 3%, mass fraction 3% sucrose solution, Feilin reagent IV, experimental process physics experiment report, chemical experiment report, biological experiment report, experimental report format, experimental report template 5. Discussion
1. The prepared soluble starch solution must be completely cooled before use. why?
2. Why do you want to control the temperature at 60 °C when the two tubes are kept warm?
3. If the No. 2 test tube also produced a brick red precipitate, what could be caused by it?
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