Pharmacology room training summary

Xxxx years xx xx to xx days, I went to the pharmacology room for a two-week training, mainly to learn the detection of endotoxin.

Director Xiao arranged for my teacher to be Dr. Peng. It is said that the doctor's turn to which project, which project has very few inspections, I have seen it once. After staying for three days, the total amount of endotoxin test was 4 batches. In order to reduce costs, it is generally necessary to make about 10 batches to do endotoxin testing together. So I have the opportunity to learn other testing projects.

For various reasons, there is no animal room in the municipal drug testing institute, and I have not done animal experiments in my major, so I was very excited to learn that I had the opportunity to follow the test of heat source with Director Wu and Teacher Cheng. When I first entered the animal room, the rabbit was fixed. When I was doing the heat source the next day, I realized that it was so terrible to catch rabbits and fix rabbits. First, grab the rabbit out of the cage according to the number, then weigh it and put it on the test bench. Fortunately, the process of fixing the rabbit does not need me to do it. Regarding this preparatory work in the early stage of the heat source, privately think that the boys are more suitable, especially when choosing a new rabbit, the rabbit is more fierce. It is said that the comrades who caught the rabbit have had the experience of being bitten by it, and finally know what the rabbit is anxious. Still biting people.

The second animal test to study was the ear vein of a guinea pig. It is said that the ear vein of the guinea pig is the most difficult to fight. Learn this, other intravenous injections are faster. The most terrible thing is still fixed guinea pigs, and when they are caught from the cage, they are soft and hot. The guinea pigs must be shouted when they are fixed, but I am afraid when I hear them, I feel like I am killing, of course, we are not going to kill it for the time being. Although I saw more shots from nurses and sisters, I had to take a syringe to pierce the skin of a guinea pig and look for blood vessels to get in. It was still difficult. I accidentally hit the skin and then I swollen a small bag. For the first time, the guinea pig only scored 3 needles.

The third animal test is to beat the tail vein of the mouse and also to administer the stomach. The gavage is better to master; and the tail vein injection, because of the contact basis of the guinea pig ear vein injection, also feels less difficult. Compared with the ear veins, the tail vein is clearly visible, and it feels more like a hit. When it is scored, it seems to have entered a pipe. This is a thing~ I can only say it, I can’t say it.

Finally, I finally have the opportunity to learn the detection of endotoxin. First, I found some places that are different from my own. The unit used a pipette when diluting and loading. The provincial side uses a graduated straw, and does not need to wash the ear or rubber head, but uses the siphon. The principle tilts the straw and slowly sucks out the liquid. I was really surprised when I saw Director Wu doing this. Later, I thought about the truth. If I use a pipette, I always change the tip, which is more troublesome. The graduated straw is more convenient to use and can be recycled and easily sterilized.

In order to make this comparison more like a summary, the end is still to write and harvest. When doing the heat source, be sure to pay attention to the probe for measuring the body temperature of the rabbit, because it will always loosen or fall out; when doing endotoxin test, the dilution process of the endotoxin standard should be as accurate as possible, and the concentration of endotoxin is correct for subsequent reactions. great influence.