Pharmacist training summary

On November 24, xxx, I was appointed to the China National Institute for the Control of Pharmaceutical and Biological Products for further study. In two thousand months, I was successfully completed with the guidance of the teachers and the enthusiastic care of the director and teachers. Learning tasks, a lot of gains. Now make a summary, please correct me.

First, the sterility inspection method verification

Under the patient guidance of Mr. Zhang Xinmei, ixabepilone and its solvent for injection, concentrated solution and dilution of cci-779 for injection, cefminox sodium, cefotaxime sodium, tazobactam sodium for injection, ceftezole sodium, More than ten kinds of injections and raw materials such as cefotaxime sodium, cefotaxime sodium for injection, ceftazidime tazobactam sodium for injection, potassium amoxicillin sodium clavulanate, and sodium sulphate hydrochloride injection. , strain preservation, passaging and other related skills

The 117 strains of lyophilized powder were resuscitated, passaged and preserved, and mastered the skills of lyophilized powder of resuscitation strains. Through the multiple inoculation, cultivation and dilution of bacteria in the experiment, the turbidimetry method was mastered. The mold spore suspension was diluted to achieve the operation specification and proficiency, and the cfu count of the diluted bacterial solution was relatively stable, thereby ensuring the smooth progress of the verification experiment. Further mastered Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa

Third, microbiological examination, strain isolation, purification, identification and other technologies

In order to understand the microbial quality of health products on the market, under the guidance of Mr. Ma Yue, Mr. Zhang Xinmei was assisted by a market survey of the varieties of commercially available health products, designed experimental protocols, and microbiological examinations of 19 health products were adopted. Dilutions and media other than the pharmacopoeia are more conducive to microbial growth. A total of more than ten typical colonies selected from the colonies grown on the culture dish were isolated and purified, Gram staining, microscopic examination and preliminary identification. The smear microscopy of the mold spores was carried out, and the morphology was initially identified. For the genus Mycobacterium. Through the above work, the knowledge has been increased, the operation steps have been familiar, and the vision has been broadened.

Fourth, the microbial limit inspection method verification

The penicillin v potassium tablets were verified by microbial limit test methods. The translation work of the English experimental materials provided by the manufacturer was independently completed, and according to the method provided by the manufacturer, the verification experiment protocol was designed according to the Chinese Pharmacopoeia, and three parallel verification experiments were carried out by the β-endoaminease method. I have studied the basic ideas, steps, calculation of recovery rate and how to select the microbial limit test method based on the calculation results. Mastering the experimental principles, methods, procedures and experimental results of the microbial limit test method verification, it has great guiding significance for returning to the laboratory's microbial limit test experiment.

V. Bacterial endotoxin test and interference test

I learned the bacterial endotoxin test and the interference test operation from the xxx teacher, and carried out the bacterial endotoxin test of four different specifications of ceftazidime tazobactam sodium for injection and sodium sulphate hydrochloride injection.

After two months of study, I got a lot of gains. Thanks to the guidance and trust of xxx teacher, let me let me complete the experiment independently, so that I can exercise my ability to think and solve problems, and the theoretical level and business ability are greatly improved. Mr. Zhang Xinmei gave me a lot of guidance and help in terms of work attitude, self-protection awareness in the experiment, life and humanity. I would like to express my heartfelt thanks to her.

Although I only learned a day from xxx teacher, she patiently explained to me, from the experimental principle, meticulously talked about the experimental concept and operation steps of bacterial endotoxin test, and guided me to personally operate, so that I can experiment in this aspect. Skills are further improved. I would like to express my heartfelt thanks.

In addition, I would like to thank the Central Inspection Office for giving me this valuable learning opportunity. I would like to thank Director Hu and Mr. Zhang for their great support. Thanks to Teacher Ma Yue, Teacher Te Yuxiang, Teacher Li Jingyun, Teacher Liu Peng and Teacher Dai Hui for their help.